By Ph.D. Agarwal;Ms Srinivas Kamini A. Rao;Ashok

ISBN-10: 8184489013

ISBN-13: 9788184489019

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40. Reefhuis J, Honein MA, Schieve LA, Correa A, Hobbs CA, Rasmussen SA. Assisted reproductive technology and major structural birth defects in the United States. Hum Reprod. 2009;24(2):360-66. 41. Scott L. Analysis of Fertilization. In: Gardner DK, Weissman A, Howles CM, Shoham Z, editors. Textbook of Assisted Reproductive Technologies: Laboratory and Clinical Perspectives. ; 2008:207-17. 42. Spandorfer SD, Avrech OM, Colombero LT, Palermo GD, Rosenwaks Z. Effect of parental age on fertilization and pregnancy characteristics in couples treated by intracytoplasmic sperm injection.

Acrosome reaction results in morphological changes of the acrosome and is triggered by an extracellular calcium influx into the sperm cell or intracellular second messengers. The hydrolytic enzymes within the acrosome (most studied are hyaluronidase and acrosin) are responsible for digesting the extracellular matrix of the oocyte. The acrosome reaction also aids in recognition, adhesion and fusion with the oocyte. The next step in fertilization is the incorporation of the sperm into the egg cytoplasm.

Agarwal A, Prabakaran SA, Sikka SC. Clinical Relevance of Oxidative Stress in Patients with male Factor Infertility: Evidence Based Analysis. In: AUA Update Series 2007;111. 2. Agarwal A, Saleh RA, Bedaiwy MA. Role of reactive oxygen species in the pathophysiology of human reproduction. Fertil Steril 2003;79(4):829-43. 3. Anderson RA, Jr, Feathergill KA, Rawlins RG, Mack SR, Zaneveld LJ. Atrial natriuretic peptide: A chemoattractant of human spermatozoa by a guanylate cyclase-dependent pathway.

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Andrology Laboratory Manual by Ph.D. Agarwal;Ms Srinivas Kamini A. Rao;Ashok

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